Aedes aegypti L. (Diptera: Culicidae) is a mosquito broadly found in tropical and temperate areas of the world. It is the main vector of dengue, chikungunya, zika and yellow fever (urban cycle), among other viruses. Aedes aegypti immatures develop in water holding containers, and frequently use artificial containers in urban settings as larval habitat. Ovitraps are artificial oviposition sites, a tool developed for mosquito population surveillance and to assess effectiveness of control measures. The preference for different oviposition substrate materials was assessed in the field, in two localities of Salta province, northern Argentina, where dengue outbreaks are frequent. The proportion of positive traps did not differ between oviposition substrates. However, higher numbers of eggs were laid in cotton fabric and velour paper, which were better than wooden paddle and blotting paper if the aim was to maximize the numbers of eggs collected. The results also evidenced that substrate preference for oviposition did not differ between geographic regions.